Ankrd1/CARP upregulation in response to Angiotensin II stimulation.

<p><b><i>(A)</i></b> Representative images of cultured neonatal rat ventricular cardiomyocytes (NRVCs) stained with rhodamine phalloidin and 4′, 6-diamidino-2-phenylindole dihydrochloride (DAPI), and the cell size increased when treated with Angiotensin II (Ang II 1 µM) for 24h. <b><i>(B)</i></b> Hypertrophic marker atrial natriuretic peptide (ANP) detected by Real-time PCR using GAPDH as an internal control (*<i>P</i><0.05 vs. the control group). Expression changes of Ankrd1 in response to Ang II in cultured NRVCs were detected by PCR <b><i>(C)</i></b> or real-time PCR <b><i>(D)</i></b>. <b><i>(E)</i></b> Western blot analysis of time-dependent changes of CARP expression in NRVCs exposed to Ang II. *<i>P</i><0.05 vs. Ang II-untreated group, #<i>P</i><0.01 vs. Ang II 24 h group. Repeat times n = 3. Data are mean ± SEM.