Subcellular translocation of CARP in response to treatment with Ang II or Ad-Ankrd1.

<p><b><i>(A)</i></b> Expression changes of calpain 1 in response to Ang II stimulation in cultured neonatal rat ventricular cardiomyocytes (NRVCs) detected by real-time PCR, the insert represents amplification curve of calpain 1 and β-actin, *<i>P</i><0.05. <b><i>(B)</i></b> Confocal microscopic subcellular distribution of CARP (green) in response to Ang II stimulation in NRVCs. Immunostaining with F-actin (red) was used to confirm the cardiomyocytes, and DAPI (blue) was used to stain the nuclei. Bar = 10 µm. <b><i>(C)</i></b> Dose-dependent infective efficiency of Ad-Ankrd1 in cultured NRVCs detected by the green fluorescence of co-expressed EGFP. MOI: multiplicity of infection. <b><i>(D)</i></b> Western blot analysis of CARP protein levels in response to different dose of Ad-Ankrd1 infection (*<i>P</i><0.01 vs. Ad-EGFP group, n = 3). <b><i>(E)</i></b> Representative EGFP fluorescence microscopic photos of LV myocardium at 1 week after intramyocardial injection of vehicle (non-transfection), Ad-EGFP or Ad-Ankrd1 delivery. <b><i>(F)</i></b> Western blot analysis of CARP protein levels in response to intramyocardial injection of Ad-EGFP or Ad-Ankrd1 at 1 week after sham operation. <b><i>(G)</i></b> Subcellular location of forced Ankrd1 expression in Ad-Ankrd1 transfected cardiomyocytes. The green fluorescence was emitted from the report gene EGFP which was constructed in adenovirus carrying Ankrd1. Bar = 10 µm.