Decitabine promotes efficient erythroid differentiation from transduced CD34⁺ cells.

<p>(a) To evaluate decitabine effects on erythroid differentiation and transduction efficiency, we transduced human CD34⁺ cells with a GFP-expressing HIV-1 vector following 48-hour prestimulation with 0.5 µM decitabine. After 24-hour transduction (without decitabine), the cells were differentiated into erythroid cells. (b) Both %GFP and GFP intensity in GPA-positive cells gradually decreased during the erythroid differentiation. The glycophorin A-positive rates (%GPA) were increased by decitabine exposure, while equivalent %GFP was observed. We analyzed data in decitabine exposure groups, compared to no decitabine exposure.