Decitabine reduced subclonal expansion of transduced CD34⁺ cells.

<p>(a). We performed a limiting dilution of human CD34⁺ cells which were transduced with lentiviral vectors after 48-hour decitabine exposure (0.5 µM), and then cultured in 96-well plates for 3–4 weeks to assay the expansion ability of transduced cells. (b). We detected subclonal expansion of GFP-positive cells that had originated from the transduced CD34⁺ cells. (c). The expansion rates were reduced by decitabine exposure under cytokine prestimulation without IL3 (2.1% vs. 9.4%) and with IL3 (0.0% vs. 7.3%). FBS: fetal bovine serum, NA: not applicable.