Side-by-side comparison of histone purities.

<p>Histones were purified according to the RHP protocol (RHP) and according to a published protocol that started with the preparation of inclusion bodies (IB; ref. 5). Both purification procedures started from the same amount of bacteria that were grown on the same day. (A) SDS-PAGE analysis. H2A showed the weakest overexpression (Fig. S1 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104029#pone.0104029.s001" target="_blank">File S1</a>) and is consequently the least pure. M: protein marker. (B) Purities.