RNAi screening for genes integral for survival towards <i>X. nematophila</i> from genome wide analysis.

<p>(A) The effect of insulin signaling genes identified as being integral for survival towards <i>X. nematophila</i> from genome wide analysis. Survival of <i>C. elegans glp-1(e2141)</i>; empty vector control (blue) n = 100 (2 independent replicates), <i>glp-1(e2141)</i>; <i>daf-16</i> (RNAi) (red) n = 100 (2) and <i>glp-1(e2141)</i>; <i>daf-18</i> (RNAi) (green) n = 100 (2) when exposed to <i>X. nematophila</i>. (B) The effect of proteasomal complex genes identified as being integral for survival towards <i>X. nematophila</i> from genome wide analysis. Survival of <i>C. elegans glp-1(e2141)</i>; empty vector control (blue) n = 100 (2), <i>glp-1(e2141)</i>; <i>rpn-6.1</i> (RNAi) (red) n = 100 (2), <i>glp-1(e2141)</i>; <i>rpn-7</i> (RNAi) (green) n = 60 (2), <i>glp-1(e2141)</i>; <i>pbs-6</i> (RNAi) (purple) n = 100 (2), <i>glp-1(e2141)</i>; <i>rpn-10</i> (RNAi) (turquoise) n = 100 (2), <i>glp-1(e2141)</i>; <i>rpt-6</i> (RNAi) (orange) n = 100 (2) and <i>glp-1(e2141)</i>; <i>pbs-4</i> (RNAi) (light blue) n = 100 (2) when exposed to <i>X. nematophila</i>. (C) The effect of new genes identified as being integral for survival towards <i>X. nematophila</i> from genome wide analysis. Survival of <i>C. elegans glp-1(e2141)</i>; empty vector control (blue) n = 100 (2), <i>glp-1(e2141)</i>; T12G3.6 (RNAi) (purple) n = 100 (2), and <i>glp-1(e2141)</i>; <i>par-5</i> (RNAi) (orange) n = 60 (2) when exposed to <i>X. nematophila.</i>