Expression of PKD, pPKD-S744/S748 and pPKD-S916 in heart homogenates.

<p>(A) Immunoblots of PKD, pPKD-S744/748 and pPKD-S916 of heart homogenates from 10-day old <i>Ryr2^+/+/AC3-C</i> (WC) and/<i>AC3-I</i> (WI) and <i>Ryr2^ADA/ADA/AC3-C</i> (HC) and/<i>AC3-I</i> (HI) mice. Glyceraldehyde-3 phosphate dehydrogenase was the loading control. (<i>B</i>) Intensity of protein PKD bands in <i>RyR2^+/+</i> and <i>RyR2^ADA/ADA</i> hearts normalized for RyR2^+/+/AC3-C protein band intensities. (C and D) pPKD/PKD on S744/748 and S916, respectively. Data were obtained analyzing proteins from 4–5 hearts of each genotype and are the mean ± SEM of 13 (B), 13(C) and 12 (D) determinations using two way ANOVA. None of the differences were significant.