Correlation of proteasome subunit expression with myositis signatures and overlap with immune cell infiltration:

<p>Expression of all proteasome units were investigated in transcriptome data of muscle biopsies referenced in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104048#pone.0104048.s007" target="_blank">table S2</a> and were correlated with genes increased in myositis muscle tissue. The heatmap of the probeset signal intensities (A) is opposed to the heatmap of the gene by gene correlation matrix (B) in 133P array experiments. Identical analysis in 133A arrays are shown in D and E. Expression of the 1209 probesets in reference transcriptomes of purified immune cells from the blood of healthy donors served as basis for clustering by probesets and cell types (C). Identification of cell type specific transcriptional patterns in the 1209 probesets demonstrates the extent of overlap of these probesets with immune cell related transcripts. Myositis samples include IBM (red), PM (orange), DM (yellow), NM (necrotizing myopathy)/IM (inflammatory myopathy) (light green) and HD (green). Immune cell types include neutrophils (dark blue), monocytes (blue), dendritic cells (light blue), CD4+ T-cells (dark violet), CD8+ T-cells (violet), NK-cells (light violet), B-cells (cyan), and healthy muscle (moss-green). A detailed graph with all probesets labelled is presented in supplemental material (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104048#pone.0104048.s004" target="_blank">figure S4</a>).