The VAD domain of bat IRF7 is functionally conserved with that of human IRF7.

<p>A. The full length human and bat IRF7 functional domain and a truncation mutant of bat IRF7 (Δ225–331, bat_tIRF7) are illustrated schematically. (B) Deletion of the VAD domain of bat IRF7 abolishes IFN transactivation. Bat IRF7 or bat tIRF7 plasmid was transfected into HEK293T cells with mouse IFN-α4 promoter plasmid with or without bat MyD88 expression plasmid and luciferase activity was tested 30 h post-transfection. Results show mean values of two experiments and error bars represent standard errors.