Initial proliferation of M133 Tregs in the DCLN is delayed compared to M133 Tconv.

<p>(<b>A</b>) Experimental design. M133 Tregs and M133 Tconv (Treg-depleted CD4 T cells) were mixed at a 2∶1 ratio and labeled with Violet. 2.25×10⁵ cells were transferred to Thy1 congenic mice one day prior to infection with rJ2.2. (<b>B</b>) Representative plots showing proliferation of M133 Tconv and Treg. (<b>C</b>) Ratio of M133 Tconv/Treg in several organs at various times p.i. (<b>D</b>) Dot plot showing entry of M133 Treg and Tconv into the infected brain at early times p.i. Percentages of M133 Treg and Tconv after gating on transferred cells are shown. (<b>E</b>) Representative plot showing proliferation of M133 Tconv and Treg in mice infected with rJ2.2.M_Y135Q, which lacks epitope M133 expression, at day 5 p.i. (<b>F</b>) Representative plot showing CXCR3 expression by M133 Treg as they proliferate. The data are representative of four (<b>B, C, D, F</b>, three mice per time point) or one (<b>E</b>, four individual mice) independent experiments.