Functional Analysis of <i>Drosophila - Figure 11 </i> HSP70 Promoter with Different HSE Numbers in Human Cells

<p>A, Flow cytometry data on HEK293/B7/<i>Gdnf/gfp</i> and HEK293/F7/<i>Gdnf/gfp</i> cells 24 (red) and 48 h (green) after heat shock at 40 (left) and 42°C (right). B, HEK293 cells transfected with F7/<i>gdnf-gfp</i> 24 h, three days (72 h), and five days (120 h) after heat shock at 42°C and injection into the caudate-putamen (striatum) of mouse. Frontal section of the mouse brain (40 µm) at the injection site. Scale bar -0.5 mm. C, Transgenic GFP expression in not heat-shocked HEK293/F7/gfp cells in the mouse brain three days after injection of cells and endothelin-1 (indicated by the arrow) and at a distance from the injection site (the fraimed area in A is enlarged in B). Scale bar on C is 500 µm and on D 100 µm.