Fitness impact of vATPase and IMPDH silencing.

<p>A) Mosquitoes were injected with vATPase dsRNA, IMPDH dsRNA and a longevity study was conducted. Survival was assessed until all mosquitoes had perished. The mean values from three replicates are shown, with the standard error bars. Additionally survival rates were analyzed by Kaplan-Meier survival analysis with Wilcoxon test, and detailed statistical information is available in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003084#pntd.0003084.s005" target="_blank">Table S3</a>. B) Fecundity analysis was conducted after vATPase dsRNA, IMPDH dsRNA was injected. The horizontal bars represent the median value of eggs laid per female. ***; p<0.001, *; p<0.05, Mann-Whitney test. C) Fertility analysis was conducted with the eggs obtained from the fecundity assay. Hatch rates indicate the average percentage of eggs giving rise to first to second instar larvae. Mean values for hatch rates and standard errors (SE) of three biological replicates are indicated.