MacroH2A2 localization changes in embryonic fibroblasts.

<p>(A) Genome browser image for anti-HA control, MAcroH2A2 in ES cells, and MacroH2A2 in MEFs. Top panel shows chromosome 12, showing general agreement between Macro localization in ES cells and MEFs. Notably, some gene poor regions gain MacroH2A2 in MEFs relative to ES cells, as shown in two successive zooms. (B) TSS-aligned MacroH2A2 averages. All Anti-Macro mapping data are averaged for all named genes for ES cells and for MEFs, as indicated. Each trace represents two averaged replicate datasets. (C) Heatmaps showing MacroH2A2 promoter localization in ES cells and MEFs, as indicated. Note that data here are normalized relative to the average MacroH2A2 level in the corresponding cell type (rather than to genome-wide data). Genes are sorted by the difference in MacroH2A2 levels between ES cells and MEFs. Right panel shows 100 gene running window average of Macro turnover in ES cells (calculated as log2 of HA enrichment at t = 12 hrs vs/t = 3 hrs – high values represent slow turnover, low value represent rapid turnover). (D) Scatterplot of change in Macro enrichment between ES and MEFs (y axis) vs. Macro turnover in ES cells, calculated as in (C) (x axis).