Altered MacroH2A2 dynamics in MEFs.

<p>(A) Averaged HA mapping data for MEFs during an HA-MacroH2A2 induction time course. Y axis is same scale as for (B), below. (B) As in (A), but for ES cells. Same as top panel in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004515#pgen-1004515-g004" target="_blank"><b>Figure 4B</b></a>. (C) Distribution of promoter turnover behavior for MEFs and ES cells. For each cell type, we estimated turnover behavior using the log2 of promoter HA data for t = 3 divided by t = 12. For each cell type, histogram shows number of genes in each bin, from rapid turnover (negative values) to slow (positive values). Note the distinct left-shifted distribution for ES cells, characteristic of overall rapid promoter Macro dynamics. In addition to being centered on zero, the width of the distribution is tighter for MEFs, indicating less variation in promoter Macro turnover in these cells. (D) Heatmap view of HA mapping data from MEFs, sorted by inferred turnover from slow (top) to rapid (bottom). Right panel shows a 80 gene running window average of mRNA abundance (log2 of Affymetrix single channel intensity). r = 0.17 between MEF Macro turnover and mRNA abundance, as compared with r = 0.46 for ES cells (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004515#pgen-1004515-g005" target="_blank"><b>Figure 5B</b></a>).