Determination of hepatocyte biomarkers of hUCMSCs and i-Hep <i>in vitro</i>.

<p>After 14-day’s induction of hepatocyte differentiation, the treated cells were stained with immunofluorescence technique using individual human antibodies. The treated cells were positive while the untreated hUCMSCs negative of the following biomarkers: (A) hCK-18, (B) hCK-19, (C) hAFP, and (D) hALB. hCK-19 was negative in both cell types. These results confirmed that the treated cells had obtained a pattern in expressing a panel of genes which is consistent with the identity of hepatocyte lineage and could be classified as i-Hep. Nuclei were stained with Hoechst 33342. 200x.