Neuritin increases synaptophysin expression in rat primary neuron cultures.

<p>Primary cultured cortical neurons were transfected at DIV 10 with EGFP or neuritin subcloned in a flag-IRES-EGFP vector by employing a calcium phosphate method. 3 days after transfection, the cells were lysed and Western blotting analysis was performed with an anti-synaptophysin antibody. β-actin was used as a loading control. Densitometric analysis was also performed. Neuritin transfection significantly increased synaptophysin expression levels by approximately 3.6-fold (*<i>p</i><0.05, Student's <i>t</i>-test). The data are presented as the mean ±SEM (n = 5 dishes from 5 independent cultures for each group).