miR-493 inhibits the proliferation and invasion of lung cancer cells by targeting E2F1.

<p>A, The knock-down of E2F1 partly simulated the suppression lung cancer cells growth induced by the overexpression of miR-493 in 95D and H1975 cells. The growth curves demonstrated the capability of lung cells proliferation. The absorption value of cells was measured at the indicated time points in triplicate and their growth rates were recorded. B, Overexpression of exogenous E2F1 (without 3′-UTR of E2F1) rescued upon the proliferation suppression induced by miR-493 in 95D and H1975 cells. C, T The invasive properties of H1975 and 95D cells which were either knock-down or overexpression of E2F1were analyzed with a transwell assay using a Matrigel-coated chamber. The migrated cells were plotted as the average number of cells per field of view from 3 different experiments, as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102602#s4" target="_blank">Materials and Methods</a>. The data are the mean ± SD.* means p<0.05. D and E, Representative images of the assays are shown. Original magnification: ×200.