The effects of hinokitiol on the expression of DNA damage regulatory proteins.

<p><b>(A)</b> The effect of hinokitiol (5 µM) or cisplatin (25 µM) on the level of γ-H2AX phosphorylation and total p53 expression in H1975 cells, as assayed using western blots. <b>(B)</b> Assessment of hinokitiol-induced DNA damage in H1975 cells through an immunofluorescence γ-H2AX focus assay. <b>(C)</b> The effect of hinokitiol (5 µM) on the level of γ-H2AX phosphorylation and total p53 expression in lung stromal fibroblasts, as assayed using western blots. <b>(D)</b> The effect of hinokitiol (5 µM) on the level of γ-H2AX phosphorylation in H1299 cells. <b>(E)</b> The effect of hinokitiol (25 µM) or cisplatin (CDDP, 25 µM) on the phosphorylation and total level of ATM, SMC3, and p53 in H1975 cells. The expression level of each protein was quantified with the NIH ImageJ program using β-actin as a loading control.