Flotillin-2 distribution in myogenic cells during skeletal muscle differentiation.

<p>Myogenic cells were grown for 24(<b>A</b> and <b>B</b>) or 72 hours (<b>C</b>). Cells were fixed with paraformaldehyde and stained with antibodies against desmin (<b>red</b>, <b>A</b> and <b>C</b>) and flotillin-2 (<b>green</b>, <b>A, B</b> and <b>C</b>) and with the nuclear dye DAPI (<b>blue</b>, <b>A</b> and <b>C</b>). Merged images are shown in <b>A</b> and <b>C</b>. Note that with paraformaldehyde fixation it is possible to see flotillin-2 almost exclusively in mononucleated cells in vesicle-like structures (<b>B</b>) and nearly absent from myotubes (<b>A</b> and <b>C</b>). Scale bar in <b>A</b> and <b>C</b> represents 20 µm and in <b>B</b> represents 10 µm.