Osteoclastogenesis is not influenced by IL-36α treatment.

<p>(A) Quantification of human osteoclast assays from four different donors, done in two independent experiments; each of them was done in triplicates, treated with rhIL36α alone, rhIL36α plus IL36RA or rhIL36α plus recombinant receptor. (B) Quantitative real-time PCR for CtsK, IL-1RAcP and IL-36R of human osteoclast precursor cells stimulated with M-CSF and RANKL to achieve osteoclast differentiation. Relative Expression was calculated from the ratio of the gene of interest to the housekeeping gene Hsp90ab1. Data from three (CtsK) or four (IL-1RAcP and IL-36R) independent donors are shown. (C) Representative pictures and quantification of three independent murine osteoclast assay untreated (control) or stimulated with 100 ng/ml recombinant murine IL36α. (D) Quantitative real-time PCR for CtsK and TRAP of murine osteoclast precursor cells stimulated with M-CSF, M-CSF and RANKL or M-CSF, RANKL and rmIL-36α to achieve osteoclast differentiation. Data from three independent experiments are shown. Relative expression was calculated from the ratio of the gene of interest to the housekeeping gene β-actin. Graphs depict mean ±SEM.