Schematic diagram of the <i>B. pseudomallei</i> K96243 <i>bprD</i> gene and agarose gel of RT-PCR products.

<p>Schematic diagram of the genomic organization of the <i>B. pseudomallei</i> K96243 region containing <i>bprD</i> (A), and RT-PCR analysis of expression of genes in this region (B). The arrows show the position and direction of genes. The positions of RT-PCR primers are indicated by black arrows. Lane 1, RT-PCR product from <i>B. pseudomallei</i> K96243 wild-type cDNA; lane 2, negative control for wild-type or DNase-treated wild-type RNA (to evaluate contamination of wild-type RNA with gDNA); lane 3, <i>B. pseudomallei</i> K96243 <i>bprD</i> mutant cDNA; lane 4, negative control for mutant or DNase-treated <i>bprD</i> mutant RNA (to evaluate contamination of mutant RNA with gDNA); lane 5, <i>B. pseudomallei</i> K96243 wild-type genomic DNA control; and lane 5, No DNA control.