Differential expression profile of Protein Kinase-C isoforms.

<p>(<b>A</b>) Western blot analyses showing change in expression of PKC α and PKC-δ (phospho and total) in groups C, H, E, H<i>^X</i> and E<i>^R</i>. PKC-δ cleavage product (41 kD) is exclusive in group H, H<i>^X</i> and E<i>^R</i>. RPL32 was used as loading control. (<b>B</b>) Western blot analyses showing phosphorylation of PKC-δ and PKC-α in adult cardiomyocytes isolated from different experimental groups. RPL32 was used as loading control. (<b>C</b>) Western blot analyses showing changes in expression of phosphorylated and total PKC-δ and PKC-α in exercise withdrawn animals (E<i>^R</i>) rested for different time periods. RPL32 was used as loading control. (<b>D</b>) Immunofluorescence study showing expression of phospho-PKC-δ and -α in different experimental group. Tissue sections showing phospho-PKC-δ expression in panels b, f, j, n and r and phospho-PKC-α in panels b’, f’, j’, n’ and r’ (green fluorescence). Sections were counter stained with alpha sarcomeric actinin antibody (panels c, g, k, o and s for PKC-δ and panels c’, g’, k’, o’ and s’ for PKC -α; red fluorescence). Nuclei were stained with DAPI (panels a, e, i, m and q for PKC-δ and panels a’, e’, i’, m’ and q’ for PKC-α; blue fluorescence) and merged images are shown in panels d, h, l, p and t for PKC-δ and panels d’, h’, l’, p’ and t’ for PKC-α. Increased expression of phospho-PKC-δ was observed in groups H and E<i>^R</i> whereas, phospho-PKC-α expression was induced in group E and H<i>^X</i> (Scale bar = 50 µm, Magnification = 40X).