Artesunate treatment of young K/BxN mice.

<p>(<b>A</b>) Ankle thickness of 3-week old K/BxN mice were treated with artesunate for 2 weeks (100 mg/kg, twice/day). Ankle thickness is shown as mean ± SD (n = 4 per group); (<b>B</b>) serum anti-GPI titers of mice treated in (A) at 5 weeks of age, each symbol represents one mouse; (<b>C</b>) flow cytometric analysis of GC B cells by Fas and GL-7 staining; (<b>D</b>) flow cytometric analysis of Tfh cells by PD-1 and CXCR5 staining at 5 weeks. Representative of two experiments. *** = p<0.0001 (2-way ANOVA, (A)); * = p<0.05, ** = p<0.01 (Students t-test, (B–C)).