Epigenetic profile of DNMT3L DMC and the associated promoter in the reporter gene assay.

<p>A&B. Histone modifications associated with DNMT3L DMC and the hsp70 promoter in the transgene reporter assay in Drosophila. Histone ChIP analysis for the 3L-L region (A) and the CMV promoter (B) in the Drosophila transgene assay. Chromatin immunoprecipitation was carried out on the 25.2.12 transgenic line with the indicated histone H3 modifications, followed by quantitative Real-time PCR. Comparison of histone modifications associated with the hsp70 promoter in the 3L-L (3LL, black bars) transgenic lines and their flipped out counterparts (Δ3LL, white bars). The H3 histone modifications examined are mentioned below the X-axis. Enrichment in the bound fraction is represented as percentage of Input. IgG - control ChIP with rabbit IgG. Error bars represent Standard Deviation (S.D.). Asterisks indicate significant difference (Student's t test, * - p<0.05, ** - p<0.01, *** - p<0.005).