Cereulide disrupts normal mitochondrial structure in MIN6 cells.

<p>Electron microscopic evaluation of MIN6 cells in control condition (A) or after 24 h exposure to 0.5 ng/ml cereulide (B) (magnification 2500x). Arrows indicate normal mitochondria in control condition (A) or enlarged mitochondria with less cristae after cereulide exposure (B). Morphometric analysis confirmed fewer mitochondria (D) and swelling of the remaining mitochondria (E) in the exposed cells, as is calculated by dividing the mitochondrial area by the number of mitochondria. In the cereulide exposed condition, we noted cells with chromatin condensation (indicated by CC in panel 4C), compared to normal nuclei (indicated by NC in panel A). After cereulide exposure there were more autophagic vacuoles (F, indicated by white arrow in panel C) Data are presented as mean ± SEM. * P<0.05 vs control; *** P<0.001 vs control (student t test).