Effects of RITA on p53 signaling in head and neck cancer cells.

<p>(A and B) HN30 (wild-type p53) and HN31 (mutated p53) head and neck cancer cells were treated with RITA for the indicated times (A) and doses (B) and the expression of p53 and its targets were evaluated by immunoblotting. (C) Cells were treated with RITA (0.1 µM–20 µM) for 72 hours and assessed via MTT assay. Data are expressed as means ± S.E from three experiments. (D) HN30 and HN31 cells were treated with RITA at the indicated doses for 10–14 days, after which colonies were fixed, stained, and quantified. Representative images from three experiments with similar results are shown. Quantitative data are expressed as means ± S.E from three experiments. * - indicates p<0.05 versus untreated control.