HA and NA properties of H1N1pdm HA222 variants.

a<p>Dissociation constant Kd of the viruses for 6′SLN sialylglycopolymer (Neu5Acα2-6Galβ1-4GlcNAc-PAA-Biotin) and 3′SL sialylglycopolymer (Neu5Acα2-3Galβ1-4Glc-PAA-Biotin) were calculated using the non-linear one site specific binding equation using the GraphPad software. The values are the mean of two assays.</p>b<p>Michaelis Menten constants (Km) were determined with MU-NANA ranging from 10 to 200 µM. Results are given as the mean Km values calculated using values derived from three separate assays.</p>c<p>Neuraminidase inhibition assays (IC50) were performed on cell supernatants with MU-NANA as a substrate at a final concentration of 50 µM. Values were obtained from duplicate samples.