Impairment of BM hematopoietic niche during GvHD.

<p>(A) Re-transplantation: In order to evaluate the effects of GvHD on BM niche, recipient mice in the GvHD and BMT groups received a second transplantation from healthy C57BL/6 BM cells (CD45.2, 5×10⁵) after 200cGy TBI on days 14 after first transplantation. Hematopoiesis was analyzed on day 14 after re-transplantation. (B) Count of MNCs per tibia (left bars) and the percentage of C57BL/6 donor-derived CD45.2⁺ cells in MNCs (right bars). (C) Absolute number of donor-derived B cells (CD45.2⁺/B220⁺), monocytes (CD45.2⁺/CD11b⁺) and granulocytes (CD45.2⁺/Gr-1⁺). (D) Percentages of donor-derived B cells, monocytes and granulocytes in MNCs. Data are shown as mean ± SD and from 1 of 3 experiments with similar results. *<i>P</i><0.05; **<i>P</i><0.01; NS: no significant (n = 4, <i>t</i>-test).