Recognition of ETEC K88 to the size-exclusion chromatography (SEC) fractions obtained.

<p>A) SEC of the >300-kDa fraction. Distribution of the eight fractions obtained. B) Number of bacteria (log CFU per well) attached to wells coated with the different fractions in the <i>in vitro</i> adhesion test (AT). The higher the log CFU counts than those of PBS, the higher the adhesive ability. C) Number of bacteria (log CFU per well) that attached to IPEC-J2 cells in the blocking test (BT). The lower the log CFU counts than those of PBS, the higher the blocking-adhesion ability. In Figures B and C, different letters mean significant differences (P<0.05) between fractions. Data result from two independent assays performed in triplicate. Error bars represent the standard error of the mean. D) Dot-blot analysis with purified fimbriae on immobilized WB extract, >300-kDa and <300-kDa fractions, CGMP, BSA, fetuin and the eight fractions (F1 to F8). (i) Binding of purified K88ac fimbriae of ETEC strain FV12408. (ii) Binding of purified K88ac fimbriae of ETEC strain 5/95. E) One-dimension SDS-PAGE of the eight fractions obtained by SEC.