Characteristics of TDLU and CCH, and laser capture microdissection.

<p>Tissues were fixed in formalin, embedded in paraffin, sectioned and immunohistochemically stained with hematoxylin and eosin. Selected specimens were stained for ERα as additional control for the selection of the lesions. <i>A)</i> Identification of TDLU (arrow head) and CCH (arrow) based on morphology (microscopic evaluation) (20X). <i>B)</i> ERα expression in TDLU and CCH (20X). <i>C–H)</i> Images of TDLU (upper panel <i>C–E</i>) and CCH (lower panel <i>F–H</i>) before (<i>C, F)</i> and after laser capture microdissection (LCM) of epithelia (<i>D, G</i>) and stroma (<i>E, H</i>) (40X).