Representative phase contrast microscopy analysis of cultured cells.

<p>(A): top, parental cell lines (LI, U87 and U373 cells) cultured in their standard medium added of 10% serum. Middle, formation of neurospheres on top of the monolayers of U373 cell line. Images were taken after 1, 2 and 3 days in neurosphere medium. Bottom, images of primary NS generated by LI, U87 and U373 cells by day 8. (B) Clones derived from LI (D2 and F11) and U87 (C7 and E8) primary NS. Original magnification 200× or 400×. Scale bar = 100 µm.