EBNA3C suppresses poly-ubiquitination of Pim-1.

<p>A–B) 10 million HEK-293 cells were transiently transfected with different combinations of expression vectors as indicated. Cells were harvested after 36 hrs of post-transfection by incubating with proteasome inhibitor MG132 drug for additional 6 hrs. Total protein was immunoprecipitated (IP) with indicated antibodies and protein samples were resolved by 10% SDS-PAGE. Western blots were performed by stripping and re-probing the same membrane. C–D) 50 million EBV negative BJAB cells, BJAB10, LCL1, sh-Ctrl, sh-Pim-1 LCL1 cells were incubated with proteasome inhibitor MG132 drug (20 µM) for 6 hrs. Cells were harvested and lysed with RIPA buffer. IRF4 was immunoprecipitated (IP) by using specific antibodies. Samples were resolved by 10% SDS-PAGE. Western blotting (WB) was performed by stripping and re-probing the same membrane.