EVM005 is required for virulence during ECTV infection of C57BL/6 and A/NCR mice.

<p>(A and B) Female C57BL/6 mice were mock-infected or infected with 10,000 pfu of ECTV, ECTV pfu of ECTV, ECTV-Δ005, ECTV-005-rev or ECTV-005(1-593)-rev via intranasal inoculation. Mice were monitored daily for mortality, day of death (A), and body weight (B). (C and D) Alternatively, female A/NCR mice were mock-infected or infected with 10,000 pfu of ECTV, ECTV pfu of ECTV, ECTV-Δ005, ECTV-005-rev or ECTV-005(1-593)-rev via footpad injection. Mice were monitored daily for day of death and mortality (C) as well as body weight (D). Morality curves were statistically analyzed using a Log-rank (Mantel-Cox) test.