Y120 and Y129 of M2 are required for expansion of primary murine B cells and for IL-10 production.

<p>(A and B) Primary murine B cells were transduced with retroviral vectors encoding Y120F or Y129F mutations of M2, wild type M2 or M2stop as a negative control. On days 2–5 post transduction, the cells were analyzed by (A) flow cytometry to monitor Thy1.1 expression, and (B) the supernatants from cells in (A) were analyzed for IL-10 secretion by ELISA. (C) 1–2×10⁶ cells each of the Y120F or Y129F inducible cell lines described in materials and methods were induced with doxycycline for 48 hours and supernatants were harvested for measurement of IL-10 secretion by ELISA. Data shown is a representative of one experiment with three replicates per condition. Each experiment was performed at least twice with at least three independent replicates per experiment.