Y129, but not Y120, of M2 is required for differentiation of infected B cells to plasma cells.

<p>(A–D) C57BL6 mice were infected with 1000PFU of the indicated virus either intranasally (A and C) or intraperitoneally (B and D) and splenocytes were harvested at indicated time points. Splenocytes were stained for flow cytometry as described in materials and methods. (A and B) Mice were harvested at the indicated time points after either IN (A) or IP (B) inoculation. YFP positive cells with a germinal center phenotype were analyzed as follows- doublet discriminated cells were gated on dump⁻ (a mix containing CD3, CD4 and CD8 antibodies to gate out T cells)/B220⁺/YFP+/CD95⁺GL7⁺. Data shown represents the frequency of Dump⁻/B220⁺/YFP⁺ cells that are CD95⁺GL7⁺, representing the infected cells with a germinal center phenotype. (C and D) Mice were harvested at the indicated time points after either IN (C) or IP (D) inoculation. YFP positive cells with a plasma cell phenotype were analyzed as follows- doublet discriminated cells were gated on dump⁻ (a mix containing CD3, CD4 and CD8 antibodies to gate out T cells)/YFP+/B220^l°CD138^hi. Data shown represents the frequency of Dump⁻/YFP⁺ cells that are B220^l°CD138^hi representing the infected cells with a plasma cell phenotype. Each data point refers to an individual mouse and the plots depict total mice from 3–4 experiments with 3–5 mice per group per experiment. <i>P</i> values were determined using a two-tailed paired Student’s <i>t</i> test (n.s., not significant).