Fiber type-specific nuclear and cytosolic PGC-1α content in the rat plantaris muscle.

<p>Representative nuclei (A) and PGC-1α (B) immunolabeling of an individual fiber (traced and isolated using ImageJ). (C–D) Surface plot of nuclei (C) and PGC-1α (D) of the images shown in (A) and (B) respectively. (E–F) Quantifications of the fiber type-specific nuclear (E) and cytosolic (F) PGC-1α content (performed on 9 fibers for each fiber type). (G) Nuclear to cytosolic PGC-1α ratio. (E–F–G) Fiber types that do not share the same letter are significantly different (p<0.05). Statistical comparisons were performed using a one-way anova and a Tukey’s post hoc test.