Uptake of mannosylated VLP in human APCs is dependent on mannose receptor association.

<p>Human monocyte-derived DCs and macrophages were left untreated (white bars) or treated with 3 mg mL⁻¹ mannan (grey bars) before a 1 hour incubation with DyLight labeled; VLP, monomannose-VLP (M-VLP) or dimannose-VLP (D-VLP) then analyzed by flow cytometry to determine the mean florescence intensity (MFI) of VLP-DyLight in the different APCs. (A–D) Graphs depict MFI of cells from four independent experiments ± S.E.M. Effect of mannan on VLP binding (4°C) to DCs (A) and macrophages (C). Effect of mannan on the uptake (37°C) of VLP by DCs (B) and macrophages (D). Statistical significance displayed is the comparison between untreated cells and mannan treated cells as determined by matched two-way ANOVA with Bonferroni post-hoc tests, ***p<0.001, **p<0.01, *p<0.05, NS = Non-significant.