Enhanced mannosylated VLP uptake is due to mannose receptor association.

<p>Murine splenocytes were left untreated (white bars) or treated with 3 mg mL⁻¹ mannann (grey bars) before a 1 hour incubation with DyLight labeled; VLP, monomannose-VLP (M-VLP) or dimannose-VLP (D-VLP) then analyzed by flow cytometry to determine the mean florescence intensity (MFI) of VLP-DyLight in the different APCs. (A) Cell Viability, as determined by the live dead yellow stain. (B–E) Graphs depict MFI of cells from 3 or 4 independent experiments ± S.E.M. Effect of mannan on VLP binding (4°C) to DCs (B), macrophages (D) and B cells (F). Effect of mannan on the uptake (37°C) of VLP by DCs (C), macrophages (E) and B cells (G). Statistical significance displayed is the comparison between untreated cells and mannan treated cells as determined by matched two-way ANOVA with Bonferroni post-hoc tests, ***p<0.001, **p<0.01, *p<0.05, NS = Non-significant.