Multiple DC subsets elicit IFN-γ production by CD4⁺ but not CD8⁺ T_EFF cells.

<p>(<b>A</b>) Sorting strategy used to isolate CD11c⁺CD11b^lo and CD11c⁺CD11b^hi DCs, CD11c⁻Ly6C^int neutrophils (Neut) and CD11c⁻Ly6C^hi monocytes (Mono) from skin 5 days after HSV-1 infection. (<b>B</b>,<b>C</b>) Analysis of IFN-γ⁺ cells after culture of <i>in vitro</i> activated gDT-II and gBT-II effector cells with 5×10⁴ (<b>B</b>) or increasing numbers (<b>C</b>) of sorted APCs pooled from 10–20 mice. Culture period, 5 h for gBT-I and 18 h for gDT-II cells. Data pooled from 2–3 experiments. (<b>D</b>,<b>E</b>) Analysis of IFN-γ⁺ T_EFF cells cultured with indicated DC subsets pooled from 10–20 mice and sorted from skin (<b>D</b>; 1.5×10⁴) or axillary LNs (<b>E</b>; 5×10⁴) 5 days after infection as in <b>B</b>,<b>C</b>. LC, Langerhans cells. Representative plots from 2–3 independent experiments.