MHC-dependent activation of T_EFF cells by APCs.

<p>(<b>A</b>) Mice received naïve gBT-I or gDT-II cells prior to HSV-1 skin infection and DNFB treatment on opposite flanks. Analysis of IFN-γ⁺ gBT-I and gDT-II cells from skin (collagenase digestion) 5 days post-infection. Data representative of <i>n</i> = 2 experiments with skin pooled from 4 mice each. (<b>B</b>–<b>D</b>) Wild-type (WT) and H-2K^b−/− mice or WT→WT and H-2K^b−/−→WT bone marrow chimeric mice were subjected to HSV-1 skin infection and transferred with <i>in vitro</i> activated gBT-I cells 3 days post-infection. (<b>E</b>) WT→WT and WT→H-2K^b−/− mice received naïve gBT-I cells prior to infection. (<b>B</b>–<b>E</b>) Analysis of IFN-γ⁺ gBT-I cells from axillary LN (<b>B</b>) and epidermis (Epi, dispase digestion, <b>C</b>–<b>E</b>) 5 days post-infection. **, <i>P</i><0.01; ***, <i>P</i><0.001; ns, not significant by Mann Whitney test; <i>n</i> = 7–14 mice/group from 3–5 experiments. (<b>F</b>,<b>G</b>) WT and I-A/E^−/− mice were subjected to HSV-1 skin infection and transferred with <i>in vitro</i> activated gDT-II cells 3 days later. Analysis of IFN-γ⁺ gDT-II cells from skin (collagenase digestion) and axillary LNs 5 days post-infection. *, <i>P</i><0.05; **, <i>P</i><0.01 by Mann Whitney test; 3 experiments with <i>n</i> = 2–4 mice/group; symbols for skin represent values from pooled tissues. (<b>H</b>) WT→WT and WT→I-A/E^−/− mice received naïve gDT-II cells prior to infection. Analysis of IFN-γ⁺ gDT-II cells in skin (collagenase digestion) and axillary LNs 5 days post-infection. *, <i>P</i><0.05 by Mann Whitney test; <i>n</i> = 9–19 mice/group from 3 experiments.