Epidermal APCs trigger IFN-γ production by CD8⁺ T_EFF cells.

<p>(<b>A</b>,<b>B</b>) Analysis of IFN-γ⁺ <i>in vitro</i> activated gBT-I (<b>A</b>,<b>B</b>) and gDT-II (<b>B</b>) T_EFF cells cultured (as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004303#ppat-1004303-g005" target="_blank"><b>Figure 5</b></a>) with increasing numbers of CD45.2⁻ and CD45.2⁺ APCs sorted from epidermal sheets (pooled from 20 mice) 4 days after HSV-1 skin infection. Data representative (<b>A</b>) or pooled from 2–3 experiments (<b>B</b>). (<b>C</b>,<b>D</b>) Analysis of IFN-γ⁺ <i>in vitro</i> activated gBT-I cells cultured with sorted APCs from epidermal sheets (pooled from 20 mice) 4 days after infection. APC numbers were 5×10⁴ for keratinocytes (KC), CD11c⁺ and residual CD45.2⁺CD11c⁻Vγ3⁻ (CD45.2⁺) cells; 3×10⁴ for DETCs. Data representative (<b>C</b>) or pooled from 2 experiments (<b>D</b>). **, <i>P</i><0.01 by student t-test individually comparing specific APC subsets to control conditions (No APC).