Distribution of IFN-γ⁺ T_EFF cells.

<p>Mice received naïve gBT-I or gDT-II cells prior to HSV-1 skin infection. (<b>A</b>) Quantification of T_EFF cells in epidermis (Epi) and dermis (Der) 5–10 days post-infection, tracked as CD45.2⁺CD45.1⁺ Vα2⁺ (gBT-I) or Vα3.2⁺ (gDT-II) cells (dispase digestion). Data pooled from <i>n</i> = 9–14 mice/time point. (<b>B</b>,<b>C</b>) Analysis of IFN-γ⁺ T_EFF cells in skin (dispase digestion) 5 days post-infection. **, <i>P</i><0.01; ns, not significant by Mann Whitney test; <i>n</i> = 5–6 mice/group from 3–4 experiments. (<b>D</b>) Analysis of IFN-γ⁺ T_EFF cells 5–10 days post-infection; <i>n</i> = 4–7 mice/time point from 1–4 experiments. (<b>E</b>,<b>F</b>) Analysis of IFN-γ⁺ T_EFF cells in skin (gBT-I, epidermis; gDT-II, total skin), brachial (brLN) and axillary (axLN) LNs and spleen (5 days post-infection in <b>E</b>, or as indicated in <b>F</b>); <i>n</i> = 2–7 mice/organ/time point from 1–4 experiments.