Comparison between methylation levels within igfbp3 promoter in crc tissues.

<p>(a) Schematic overview of the insulin-like grown factor binding protein 3 (igfbp3) gene. exons are represented by blue boxes, introns with blue lines, and the promoter region by blue dashed lines. the <i>igfbp3</i> gene has one cytosine-phosphate-guanine (cpg) island at in the promoter region and exon1 underscoring the potential of modulating gene expression by means of cpg hypermethylation, and this is represented by a green box. we performed two different assays covering both cpgs islands (indicated by green arrows) using two quantitative methods: pyrosequencing and qmsp in order to correlate methylation levels of these two locations. (b) Linear regression analysis comparing <i>igfbp3</i> methylation levels by pyrosequencing (% methylation) in 348 patient samples and qmsp (pmr) assays in crc tissues (n = 197).