Analysis of corpus cavernosum tissue fibrosis in BCN rats.

<p>(<b>A</b>) Penis samples were prepared for the detection of corpus cavernosum tissue fibrosis using Masson trichrome staining (Upper-line: 40×, Scale bars = 500 µm, down-line: 100×, Scale bars = 200 µm). (<b>B</b>) Semi-quantitative image analysis of collagen/muscle ratio in corpus cavernosum tissues was performed using the Image-Pro Plus 6.0 software. **<i>P</i><0.01 compared with the sham rats (n = 5/group). (<b>C</b>) Western blot analyses measuring collagen-I protein expression levels in penile tissues of both groups (Sham group: n = 3, BCN group: n = 4). All results are representative of three independent experiments. BCN = bilateral cavernous neurectomy; Sham = sham operation.