Effects of the chloroquine on (R/W)9-ASPNA internalization.

<p>(A) PPT/HeLa cells were incubated with 1 µM (R/W)9-ASPNA-TR with (+CQ) or without chloroquine (–CQ) for 4 h prior to microscopic observation. Images are the projection of all z-stacked signals (0.2 µm between each image). Light field (upper panels) and fluorescent images (lower panels) are shown. Scale bar: 30 µm. (B) Representative FACS data of cell uptake (TR fluorescence) of ASPNA-TR in the presence of CQ (red curve) or in the absence of CQ (black curve) and of (R/W)9-ASPNA-TR in the presence of CQ (yellow curve) or in the absence of CQ (blue curve). Fluorescence quantification (mean ± SD; n = 5) is shown in the histogram.