Immunohistochemical localization of elastin and collagen X in the scaffold-free, engineered tissue constructs.

<p>Immunohistochemical localization of elastin (red) (A, C, E, G) and collagen X (green) (B, D, F, H) in the engineered elastic cartilaginous constructs generated from monolayer preparations after 4 weeks of bioreactor culture compared to native auricular cartilage. Native auricular cartilage (A, B), Control (C, D), IGF-1 (E, F), and Insulin (G, H). Letters “P” and “C” refer to the perichondrial-like and cartilaginous regions, respectively. Scale bar: 30 µm.