Spo24^d67 localizes to the prospore cortex.

<p>(<b>A</b>) <i>spo24</i>Δ cells carrying a plasmid (pSH112) expressing Spo24-GFP were sporulated on SPM plates for 24 hours. GFP fluorescence microscopy (right panel) revealed ring-like structures that appear to correspond with spore locations in the DIC image left panel). (<b>B</b>) As in (A), except that the cells carrying a plasmid (pRS426_RFP_ Spo20^51–91 Spo24_GFP) expressing both RFP-Spo20^51–91 (a prospore marker) and Spo24-GFP. The GFP ring-like structures co-localize with the prospore marker protein (in red, merge represented by yellow), suggesting that Spo24 is associated with the prospore cortex.