Distribution of EC958 mobile genetic elements in <i>E. coli</i>.

<p><b>A.</b> Visualisation of the EC958 genome compared with three <i>E. coli</i> ST131 genomes and 16 other <i>E. coli</i> genomes using BLASTn. EC958 prophage (Phi1 – Phi7) and genomic islands (GI-<i>thrW</i>, GI-<i>pheV</i>, GI-<i>selC</i>, GI-<i>leuX</i>) are represented by black boxes in the outermost circle. The innermost circles represent the GC content (black) and GC skew (green/purple) of EC958. The remaining circles display BLASTn searches against the genome of EC958. <b>B.</b> A BRIG visualisation of the EC958 mobile elements compared with the 19 <i>E. coli</i> genomes. BLASTn searches of the 19 genomes against the EC958 prophage and genomic islands show that the EC958 GIs and prophage are well conserved in the ST131 clade C genomes but largely absent from the genomes of SE15 and the other 16 <i>E. coli</i> genomes, which are arranged inner to outer as follows: Group E strains O157 EDL933, O157 Sakai (blue); group B1 strains SE11, IAI1 (aquamarine); group A strains BW2952, MG1655, W3110, HS (green); group D strains UMN026, IAI39 (yellow); group B2 strains APEC-01, S88, 536, UTI89, CFT073, ED1A (orange); group B2 ST131 strains SE15, NA114, JJ1886, EC958 (red). Figure prepared using BRIG <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104400#pone.0104400-Touchon1" target="_blank">[28]</a>.