Left ventricular work and protection signaling in Intralipid®- and sevoflurane-treated early type-2 diabetic hearts.

<p><u>Panel A</u>: average left ventricular work (LVW) during equilibration (striped columns) and reperfusion (30 min: solid columns) in untreated early diabetic hearts (ff-IR: N = 10), early diabetic hearts exposed to 2 vol.-% sevoflurane (ff-IR/SEV; N = 10), and early diabetic hearts treated with 1% Intralipid® at the onset of reperfusion (ff-IR/IL; N = 6). Data are mean ± SD. <u>Panel B</u>: p-Akt (at Ser473) to total Akt immunoblots from tissue samples collected 3 min and 10 min after the onset of reperfusion. <u>Panel C</u>: p-ERK1/2 (at Thr202/Tyr204) to total ERK1/2 immunoblots from the same tissues. ff-IR(time), untreated hearts exposed to 15 min of ischemia and 3 min (ff-IR/3 min) or 10 min (ff-IR/10 min) of reperfusion, respectively; ff-IR/SEV(time), hearts exposed to ff-IR(time) and 2 vol.-% sevoflurane; ff-IR/IL(time), hearts exposed to ff-IR(time) and 1% Intralipid® at the onset of reperfusion. *, significantly different from ff-IR(time); **, significantly different from ff-IR(time) and ff-IR/IL(time); #, significantly different from ff-IR/IL(time). Data are mean ± SD. N = 4 hearts in each group.