Mst1 is required for integrin-independent T cell polarization.

<p><b>A</b>) Wt and <i>Mst1^h/h</i> CD4 T cells were stimulated with 100 ng/mL CCL19 in PBS for 30 minutes. Polarization of CD44 to the uropod and LFA-1 distribution were visualized by confocal microscopy. <b>B</b>) Computational scoring of CD44 and LFA-1 clustering on wt and <i>Mst1^h/h</i> CD4 T stimulated with 100 ng/mL CCL19 in PBS prior to fixation and staining for LFA-1 and CD44 expression. Student's t-test was performed to compare clustering efficiency for Mst1^wt and Mst1^h/h T cells.